In the city of London, a young girl named Espella Cantabella is chased after by a mysterious force and seeks the aid of Professor Layton and his assistant, Luke Triton. When Espella is suddenly captured, Layton's investigation leads him to a strange book which transports him and Luke to a strange medieval city known as Labyrinthia. This town is allegedly home to witches who hides in the shadows, and is controlled by someone called the Story Teller, who allegedly has the power to turn anything he writes into reality. Meanwhile, Phoenix Wright and his assistant, Maya Fey, who have traveled to London on business, encounter the same book and are also transported to Labyrinthia. There, they discover Espella has been accused of being a witch and is to be put on trial. As Phoenix decides to defend Espella, soon discovering that the laws of this world are far different from his own, Layton seeks to solve the mystery behind Labyrinthia, with the two not always seeing eye to eye.